Title | DNA-CNT nanowire networks for DNA detection. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Weizmann, Y, Chenoweth, DM, Swager, TM |
Journal | Journal of the American Chemical Society |
Volume | 133 |
Pagination | 3238–41 |
Date Published | mar |
ISSN | 1520-5126 |
Keywords | Carbon, Carbon: chemistry, DNA, DNA Probes, DNA Probes: chemistry, DNA: analysis, Electric Conductivity, Electron, Humans, Microscopy, Nanotubes, Nanowires, Nanowires: chemistry, Nucleic Acid Hybridization, Nucleic Acid Hybridization: methods, Scanning |
Abstract | The ability to detect biological analytes in a rapid, sensitive, operationally simple, and cost-effective manner will impact human health and safety. Hybrid biocatalyzed-carbon nanotube (CNT) nanowire-based detection methods offer a highly sensitive and specific platform for the fabrication of simple and effective conductometric devices. Here, we report a conductivity-based DNA detection method utilizing carbon nanotube-DNA nanowire devices and oligonucleotide-functionalized enzyme probes. Key to our sensor design is a DNA-linked-CNT wire motif, which forms a network of interrupted carbon nanotube wires connecting two electrodes. Sensing occurs at the DNA junctions linking CNTs, followed by amplification using enzymatic metalization leading to a conductimetric response. The DNA analyte detection limit is 10 fM with the ability to discriminate single, double, and triple base pair mismatches. DNA-CNT nanowires and device sensing gaps were characterized by scanning electron microscopy (SEM) and confocal Raman microscopy, supporting the enhanced conductometric response resulting from nanowire metallization. |
URL | http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3065376&tool=pmcentrez&rendertype=abstract |
DOI | 10.1021/ja109180d |