Strain release in organic photonic nanoparticles for protease sensing.

TitleStrain release in organic photonic nanoparticles for protease sensing.
Publication TypeJournal Article
Year of Publication2012
AuthorsCordovilla, C, Swager, TM
JournalJournal of the American Chemical Society
Date Publishedapr
KeywordsCross-Linking Reagents, Cross-Linking Reagents: chemistry, Luminescence, Molecular Structure, Nanoparticles, Nanoparticles: chemistry, Peptide Hydrolases, Peptide Hydrolases: chemistry, Peptide Hydrolases: metabolism, Photons, Polymers, Polymers: chemistry

Proteases are overexpressed in most cancers and proteolytic activity has been shown to be a viable marker for cancer imaging in vivo. Herein, we describe the synthesis of luminescence-quenched shell cross-linked nanoparticles as photonic nanoprobes for protease sensing. Protease sensing scheme is based on a \"turn-on\" mechanism where the protease cleaves peptide cross-linkers of the fluorescence-quenched shell cross-linked NP (OFF state) leading to a highly emissive non-cross linked NP (ON state). The cross-linked particles can be strained by exposure to a good solvent and proteolysis allows for particle expansion (swelling) and a recovery of the luminescence.