Detection of myelination using a novel histological probe.

TitleDetection of myelination using a novel histological probe.
Publication TypeJournal Article
Year of Publication2005
AuthorsXiang, Z, Nesterov, EE, Skoch, J, Lin, T, Hyman, BT, Swager, TM, Bacskai, BJ, Reeves, SA
JournalJournal of Histochemistry and Cytochemistry
Date Publisheddec
KeywordsAnimal, Animals, Brain, Brain: metabolism, Demyelinating Diseases, Demyelinating Diseases: metabolism, Disease Models, Fluorescence, Fluorescent Dyes, Fluorescent Dyes: chemical synthesis, Fluorescent Dyes: chemistry, Humans, Immunohistochemistry, Inbred C57BL, Mice, Myelin Sheath, Myelin Sheath: metabolism, Near-Infrared, Spectrometry, Spectroscopy

Current methods for myelin staining in tissue sections include both histological and immunohistochemical techniques. Fluorescence immunohistochemistry, which uses antibodies against myelin components such as myelin basic protein, is often used because of the convenience for multiple labeling. To facilitate studies on myelin, this paper describes a quick and easy method for direct myelin staining in rodent and human tissues using novel near-infrared myelin (NIM) dyes that are comparable to other well-characterized histochemical reagents. The near-infrared fluorescence spectra of these probes allow fluorescent staining of tissue sections in multiple channels using visible light fluorophores commonly used in immunocytochemistry. These dyes have been used successfully to detect normal myelin structure and myelin loss in a mouse model of demyelination disease.