High field dynamic nuclear polarization NMR with surfactant sheltered biradicals.

TitleHigh field dynamic nuclear polarization NMR with surfactant sheltered biradicals.
Publication TypeJournal Article
Year of Publication2014
AuthorsKiesewetter, MK, Michaelis, VK, Walish, JJ, Griffin, RG, Swager, TM
JournalThe journal of physical chemistry. B
Volume118
Pagination1825–30
Date Publishedfeb
ISSN1520-5207
Keywordsnucleus polarization NMR spectroscopy surfactant b
Abstract

We illustrate the ability to place a water-insoluble biradical, bTbk, into a glycerol/water matrix with the assistance of a surfactant, sodium octyl sulfate (SOS). This surfactant approach enables a previously water insoluble biradical, bTbk, with favorable electron-electron dipolar coupling to be used for dynamic nuclear polarization (DNP) nuclear magnetic resonance (NMR) experiments in frozen, glassy, aqueous media. Nuclear Overhauser enhancement (NOE) and paramagnetic relaxation enhancement (PRE) experiments are conducted to determine the distribution of urea and several biradicals within the SOS macromolecular assembly. We also demonstrate that SOS assemblies are an effective approach by which mixed biradicals are created through an assembly process.

URLhttp://www.ncbi.nlm.nih.gov/pubmed/24506193
DOI10.1021/jp410387e