|Title||Strain release in organic photonic nanoparticles for protease sensing.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Cordovilla, C, Swager, TM|
|Journal||Journal of the American Chemical Society|
|Keywords||Cross-Linking Reagents, Cross-Linking Reagents: chemistry, Luminescence, Molecular Structure, Nanoparticles, Nanoparticles: chemistry, Peptide Hydrolases, Peptide Hydrolases: chemistry, Peptide Hydrolases: metabolism, Photons, Polymers, Polymers: chemistry|
Proteases are overexpressed in most cancers and proteolytic activity has been shown to be a viable marker for cancer imaging in vivo. Herein, we describe the synthesis of luminescence-quenched shell cross-linked nanoparticles as photonic nanoprobes for protease sensing. Protease sensing scheme is based on a \"turn-on\" mechanism where the protease cleaves peptide cross-linkers of the fluorescence-quenched shell cross-linked NP (OFF state) leading to a highly emissive non-cross linked NP (ON state). The cross-linked particles can be strained by exposure to a good solvent and proteolysis allows for particle expansion (swelling) and a recovery of the luminescence.